Rapid diagnosis of Histoplasma capsulatum endocarditis using the AccuProbe on an excised valve

R F Chemaly; J W Tomford; G S Hall; M Sholtis; J D Chua; G W Procop

doi:10.1128/JCM.39.7.2640-2641.2001

Rapid diagnosis of Histoplasma capsulatum endocarditis using the AccuProbe on an excised valve

J Clin Microbiol. 2001 Jul;39(7):2640-1. doi: 10.1128/JCM.39.7.2640-2641.2001.

Authors

R F Chemaly¹, J W Tomford, G S Hall, M Sholtis, J D Chua, G W Procop

Affiliation

¹ Department of Clinical Pathology, Section of Clinical Microbiology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.

Abstract

Histoplasma capsulatum is an infrequent but serious cause of endocarditis. The definitive diagnosis requires culture, which may require a long incubation. We demonstrated the ability of the Histoplasma capsulatum AccuProbe to accurately identify this organism when applied directly on an excised valve that contained abundant yeast forms consistent with H. capsulatum.

Publication types

Case Reports
Evaluation Study

MeSH terms

Aortic Valve
DNA Probes*
DNA, Ribosomal / analysis
Endocarditis / diagnosis*
Endocarditis / microbiology
Endocarditis / surgery
Heart Valve Prosthesis / microbiology*
Histoplasma / classification
Histoplasma / genetics
Histoplasma / isolation & purification*
Histoplasmosis / diagnosis*
Histoplasmosis / microbiology
Histoplasmosis / surgery
Humans
Male
Middle Aged
RNA, Ribosomal / genetics
Reagent Kits, Diagnostic

Substances

DNA Probes
DNA, Ribosomal
RNA, Ribosomal
Reagent Kits, Diagnostic