Objective: To study the effect of bhrfl expressing on the topoisomerase I expression in nasopharyngeal carcinoma cell line CNE2.
Methods: bhrfl recombinant expressing vector was constructed and used to transfect the nasopharyngeal carcinoma cell line CNE2. The CNE2 cells were then tested for changes of the topoisomerase I expression and anti-apoptotic ability under the treatment of camptothecin.
Results: bhrfl expressing could enhance the topoisomerase I expression in CNE2 cells, that in end reduced cell sensitivity to camptothecin increased cell survival span and decreased apoptotic rate(P < 0.01).
Conclusion: By increasing the expression of topoisomerase I, bhrfl expressing can enhance the resistance of CNE2 cells to apoptosis induced by camptothecin.