Macrophage colony-stimulating factor suppresses osteoblast formation

Biochem Biophys Res Commun. 2001 Jul 13;285(2):328-34. doi: 10.1006/bbrc.2001.5128.

Abstract

We provide the first evidence that the bone marrow-derived cytokine, macrophage colony-stimulating factor (M-CSF), inhibits the formation of bone-forming osteoblasts. We examined both osteoclast and osteoblast formation in primary rat bone marrow cultures. As expected, M-CSF together with osteoprotegerin ligand (OPGL) markedly accelerated osteoclastogenesis. In contrast, treatment with M-CSF alone yielded no osteoclasts at any time. The most striking and novel observation was that M-CSF with or without OPGL dramatically suppressed osteoblast formation. In separate experiments, estradiol markedly suppressed osteoclast formation in the M-CSF/OPGL-treated cultures independently of osteoblasts. Consistent with this was the expression of estrogen receptor-alpha (ERalpha) and ERbeta mRNA in osteoclast precursors. We therefore conclude that in addition to the well-known action of M-CSF to modulate osteoclastogenesis, this cytokine may also regulate osteoblast formation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Bone Marrow Cells / cytology*
  • Bone Marrow Cells / drug effects
  • Carrier Proteins / pharmacology
  • Cell Differentiation / drug effects
  • Cells, Cultured
  • Estrogen Receptor alpha
  • Estrogen Receptor beta
  • Gene Expression Regulation / drug effects
  • Humans
  • Kinetics
  • Macrophage Colony-Stimulating Factor / pharmacology*
  • Membrane Glycoproteins / pharmacology
  • Osteoblasts / cytology*
  • Osteoblasts / drug effects
  • Osteoblasts / physiology
  • Osteoclasts / cytology*
  • Osteoclasts / drug effects
  • Osteoclasts / physiology
  • RANK Ligand
  • Rats
  • Rats, Sprague-Dawley
  • Rats, Wistar
  • Receptor Activator of Nuclear Factor-kappa B
  • Receptors, Estrogen / genetics
  • Recombinant Proteins / pharmacology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors
  • Transcription, Genetic / drug effects

Substances

  • Carrier Proteins
  • Estrogen Receptor alpha
  • Estrogen Receptor beta
  • Membrane Glycoproteins
  • RANK Ligand
  • Receptor Activator of Nuclear Factor-kappa B
  • Receptors, Estrogen
  • Recombinant Proteins
  • TNFRSF11A protein, human
  • TNFSF11 protein, human
  • Macrophage Colony-Stimulating Factor