Abstract
The effect of huperzine A (HupA) on oxygen-glucose deprivation (OGD)-induced injury was investigated in the rat pheochromocytoma cell line PC12. OGD for 3 h and reoxygenation for 24 h triggered apoptosis characterized by chromatin condensation, nucleus fragmentation and DNA laddering. The temporal profile of c-jun, p53, bcl-2 and bax mRNA after OGD indicated that these genes played important roles in apoptosis. Pre-incubation of the cells for 2 h with 1 microM HupA significantly attenuated apoptosis. The same treatment also reduced the up-regulation of c-jun and bax as well as the down-regulation of bcl-2. These data suggest the ability of HupA to attenuate apoptosis induced by OGD may result from its capability to alter the expression of apoptosis-related genes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaloids
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Animals
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Apoptosis / drug effects
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Apoptosis / physiology
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Cell Hypoxia / drug effects
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Cell Hypoxia / physiology
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DNA Fragmentation / drug effects
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DNA Fragmentation / physiology
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Gene Expression / drug effects
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Gene Expression / physiology
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Genes, bcl-2 / drug effects
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Genes, bcl-2 / physiology
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Genes, jun / drug effects
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Genes, jun / physiology
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Genes, p53 / drug effects
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Genes, p53 / physiology
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Neuroprotective Agents / pharmacology*
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PC12 Cells
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Proto-Oncogene Proteins / metabolism
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Proto-Oncogene Proteins c-bcl-2*
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Rats
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Sesquiterpenes / pharmacology*
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bcl-2-Associated X Protein
Substances
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Alkaloids
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Bax protein, rat
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Neuroprotective Agents
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2
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Sesquiterpenes
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bcl-2-Associated X Protein
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huperzine A