Crystal structure of negative cofactor 2 recognizing the TBP-DNA transcription complex

Cell. 2001 Jul 13;106(1):71-81. doi: 10.1016/s0092-8674(01)00417-2.

Abstract

The X-ray structure of a ternary complex of Negative Cofactor 2 (NC2), the TATA box binding protein (TBP), and DNA has been determined at 2.6 A resolution. The N termini of NC2 alpha and beta resemble histones H2A and H2B, respectively, and form a heterodimer that binds to the bent DNA double helix on the underside of the preformed TBP-DNA complex via electrostatic interactions. NC2beta contributes to inhibition of TATA-dependent transcription through interactions of its C-terminal alpha helix with a conserved hydrophobic feature on the upper surface of TBP, which in turn positions the penultimate alpha helix of NC2beta to block recognition of the TBP-DNA complex by transcription factor IIB. Further regulatory implications of the NC2 heterodimer structure are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Caenorhabditis elegans
  • Crystallography, X-Ray / methods
  • DNA / chemistry*
  • DNA / metabolism
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / metabolism
  • Drosophila melanogaster
  • Histones / chemistry
  • Humans
  • Models, Molecular
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Phosphoproteins / chemistry*
  • Phosphoproteins / metabolism
  • Protein Structure, Secondary
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Static Electricity
  • TATA Box*
  • TATA-Box Binding Protein
  • Transcription Factor TFIIA
  • Transcription Factor TFIIB
  • Transcription Factors / chemistry*
  • Transcription Factors / metabolism
  • Transcription, Genetic
  • Xenopus laevis

Substances

  • DNA-Binding Proteins
  • Histones
  • Phosphoproteins
  • TATA-Box Binding Protein
  • Transcription Factor TFIIA
  • Transcription Factor TFIIB
  • Transcription Factors
  • down-regulator of transcription 1
  • DNA

Associated data

  • PDB/1JFI