Multi-faceted analyses of a highly metastatic human lung cancer cell line NCI-H460-LNM35 suggest mimicry of inflammatory cells in metastasis

Oncogene. 2001 Jul 12;20(31):4228-34. doi: 10.1038/sj.onc.1204561.

Abstract

This study established and characterized low-metastatic revertant and parental clones of a highly metastatic human lung cancer cell line, NCI-H460-LNM35 (hereafter referred to as LNM35). Expression-profiling analysis revealed that up-regulation of various proinflammatory cytokines and angiogenic chemotactic chemokines was present in LNM35. Further, while COX-2 itself is known to be inducible in inflammation, COX-2 expression levels correlated well with the capabilities of these clones for not only in vitro motility and invasion but also in vivo metastasis, and COX-2 inhibitors were shown for the first time to reduce lung cancer metastasis in vivo. These findings suggest that lung cancer cells may mimic inflammatory cells in the process of metastasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cyclooxygenase 2
  • Female
  • Gene Expression Profiling
  • Humans
  • Isoenzymes / metabolism
  • Lung Neoplasms / enzymology
  • Lung Neoplasms / genetics
  • Lung Neoplasms / pathology*
  • Membrane Proteins
  • Mice
  • Mice, Nude
  • Mice, SCID
  • Neoplasm Metastasis
  • Oligonucleotide Array Sequence Analysis
  • Phenotype
  • Prostaglandin-Endoperoxide Synthases / metabolism
  • Tumor Cells, Cultured

Substances

  • Isoenzymes
  • Membrane Proteins
  • Cyclooxygenase 2
  • PTGS2 protein, human
  • Prostaglandin-Endoperoxide Synthases