Decreased wild-type full-length Et-A and -B receptors in neuroblastoma and Ewing sarcoma cells

Med Pediatr Oncol. 2001 Jan;36(1):142-6. doi: 10.1002/1096-911X(20010101)36:1<142::AID-MPO1034>3.0.CO;2-Z.

Abstract

Background: Endothelins and their receptors, Et-A and Et-B, play an essential role in differentiation and migration of neural crest cells. Expression of endothelin receptors has been examined in neuroblastoma and Ewing sarcoma cell lines.

Procedure: RNA was amplified for Et-A and Et-B by RT-PCR. Amplified products were cloned into the expression vector pLNCX, which was used to transfect CHO cells. Binding characteristics of transfected CHO cells were examined.

Results: Full-length Et-A mRNA was identified in all cell lines, in addition to a truncated Et-A product. CHO cells expressing full-length Et-A bound to endothelin, but cells expressing truncated Et-A did not. Full length Et-B mRNA was not detected, but two smaller molecular weight products were amplified. These are as yet uncharacterised.

Conclusions: These results suggest that endothelins and their receptors may be important in the development and biology of neuroblastoma and Ewing sarcoma.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Neoplasms / genetics
  • Bone Neoplasms / metabolism
  • Bone Neoplasms / pathology*
  • CHO Cells
  • Cricetinae
  • Cricetulus
  • Endothelins / metabolism*
  • Exons / genetics
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Molecular Weight
  • Neoplasm Proteins / biosynthesis
  • Neoplasm Proteins / chemistry
  • Neoplasm Proteins / genetics*
  • Neuroblastoma / genetics
  • Neuroblastoma / metabolism
  • Neuroblastoma / pathology*
  • Protein Conformation
  • RNA Splicing
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • RNA, Neoplasm / biosynthesis
  • RNA, Neoplasm / genetics
  • Receptor, Endothelin A
  • Receptor, Endothelin B
  • Receptors, Endothelin / biosynthesis
  • Receptors, Endothelin / chemistry
  • Receptors, Endothelin / genetics*
  • Recombinant Fusion Proteins / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sarcoma, Ewing / genetics
  • Sarcoma, Ewing / metabolism
  • Sarcoma, Ewing / pathology*
  • Sequence Deletion*
  • Transfection
  • Tumor Cells, Cultured / metabolism

Substances

  • Endothelins
  • Neoplasm Proteins
  • RNA, Messenger
  • RNA, Neoplasm
  • Receptor, Endothelin A
  • Receptor, Endothelin B
  • Receptors, Endothelin
  • Recombinant Fusion Proteins