Purification, crystallization and identification by X-ray analysis of a prostate kallikrein from horse seminal plasma

A L Carvalho; J M Dias; L Sanz; A Romero; J J Calvete; M J Romão

doi:10.1107/s0907444901009805

Purification, crystallization and identification by X-ray analysis of a prostate kallikrein from horse seminal plasma

Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1180-3. doi: 10.1107/s0907444901009805. Epub 2001 Jul 23.

Authors

A L Carvalho¹, J M Dias, L Sanz, A Romero, J J Calvete, M J Romão

Affiliation

¹ Departamento de Química, Centro de Química Fina e Biotecnologia, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Monte de Caparica, Portugal.

PMID: 11468412
DOI: 10.1107/s0907444901009805

Abstract

The purification, crystallization and identification by X-ray diffraction analysis of a horse kallikrein is reported. The protein was purified from horse seminal plasma. Crystals belong to space group C2 and the structure was solved by the MIRAS method, with two heavy-atom derivatives of mercury and platinum. X-ray diffraction data to 1.42 A resolution were collected at the ESRF synchrotron-radiation source.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Crystallization
Crystallography, X-Ray
Horses
Kallikreins / chemistry*
Kallikreins / isolation & purification
Male
Models, Molecular
Molecular Sequence Data
Prostate / chemistry*
Protein Conformation
Semen / chemistry*
Sequence Homology, Amino Acid

Substances

Kallikreins