Inhibitory effects of T-cell stimulation and co-stimulation observed at high concentrations of plate-bound antibodies

S Lesage; J Charron; P Hugo

doi:10.1016/s0022-1759(01)00415-x

Inhibitory effects of T-cell stimulation and co-stimulation observed at high concentrations of plate-bound antibodies

J Immunol Methods. 2001 Sep 1;255(1-2):23-6. doi: 10.1016/s0022-1759(01)00415-x.

Authors

S Lesage¹, J Charron, P Hugo

Affiliation

¹ PROCREA BioSciences Inc., Division of Research and Development, 6100 Royalmount, H4P 2R2, Montreal, Quebec, Canada.

PMID: 11470283
DOI: 10.1016/s0022-1759(01)00415-x

Abstract

Plate-bound monoclonal antibodies (mAb) are often used as a way of stimulating lymphocytes in vitro. Our observations show that the concentrations of mAb used in functional assays in vitro must be carefully assessed before conclusions are drawn about lymphocyte activation or co-activation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies / pharmacology*
CD28 Antigens / immunology
Lymphocyte Activation / drug effects
Lymphocyte Activation / immunology*
Receptors, Antigen, T-Cell / immunology
T-Lymphocytes / drug effects
T-Lymphocytes / immunology*

Substances

Antibodies
CD28 Antigens
Receptors, Antigen, T-Cell