Identification of nucleotide sequences for the specific and rapid detection of Yersinia pestis

L Radnedge; S Gamez-Chin; P M McCready; P L Worsham; G L Andersen

doi:10.1128/AEM.67.8.3759-3762.2001

Identification of nucleotide sequences for the specific and rapid detection of Yersinia pestis

Appl Environ Microbiol. 2001 Aug;67(8):3759-62. doi: 10.1128/AEM.67.8.3759-3762.2001.

Authors

L Radnedge¹, S Gamez-Chin, P M McCready, P L Worsham, G L Andersen

Affiliation

¹ Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, California 94551, USA.

Abstract

Suppression subtractive hybridization, a cost-effective approach for targeting unique DNA, was used to identify a 41.7-kb Yersinia pestis-specific region. One primer pair designed from this region amplified PCR products from natural isolates of Y. pestis and produced no false positives for near neighbors, an important criterion for unambiguous bacterial identification.

Publication types

Evaluation Study
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Base Sequence
DNA Primers*
DNA, Bacterial / analysis*
Molecular Sequence Data
Nucleic Acid Hybridization / methods
Polymerase Chain Reaction
Species Specificity
Time Factors
Yersinia pestis / classification*
Yersinia pestis / genetics
Yersinia pestis / isolation & purification*

Substances

DNA Primers
DNA, Bacterial

Associated data

GENBANK/AF350073
GENBANK/AF350074
GENBANK/AF350075
GENBANK/AF350076
GENBANK/AF350077
GENBANK/AF350078
GENBANK/AF350079