Localization of human BRCA1 and BRCA2 in non-inherited colorectal carcinomas and matched normal mucosas

Anticancer Res. 2001 May-Jun;21(3B):2011-20.

Abstract

We characterized the expression of BRCA1 and BRCA2 in 38 sporadic colorectal carcinomas and matched normal mucosas with 9 anti-BRCA1 antibodies and 4 anti-BRCA2 antibodies, raised against several different epitopes, using immunohistochemical technique. We demonstrated an increased BRCA1 and BRCA2 staining in the apical cell pole of epithelial malignant cells and we also revealed a significant increase in BRCA1 and BRCA2 nuclear foci in tumor colorectal specimens in comparison with corresponding normal tissues. These increases in BRCA1 and BRCA2 expression may be explained by the fact that colorectal tissue is subject to very active proliferation and differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • BRCA1 Protein / biosynthesis*
  • BRCA1 Protein / chemistry
  • BRCA2 Protein
  • Case-Control Studies
  • Colon / metabolism*
  • Colon / pathology
  • Colorectal Neoplasms / metabolism*
  • Colorectal Neoplasms / pathology
  • Epitopes
  • Female
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Mucous Membrane / metabolism*
  • Mucous Membrane / pathology
  • Neoplasm Proteins / biosynthesis*
  • Neoplasm Proteins / chemistry
  • Transcription Factors / biosynthesis*
  • Transcription Factors / chemistry

Substances

  • BRCA1 Protein
  • BRCA2 Protein
  • Epitopes
  • Neoplasm Proteins
  • Transcription Factors