Abstract
To study the process of bone regeneration we examined three samples of periapical regenerative tissue obtained from two patients under a guided tissue regeneration treatment in endodontic surgery by the immunohistochemical and enzyme histochemical methods. The regenerative tissue consisted of a large number of fibroblast-like cells and a small number of mononuclear cells. Fibroblast-like cells stained positively for alkaline phosphatase and osteopontin, whereas mononuclear cells stained positively for CD4. Interleukin-4-producing cells could be detected in adjacent sections. However, interferon-y-producing cells could not be detected. These findings suggest that interleukin-4-producing cells may be one of the elements associated with success in the human bone regeneration process in vivo.
MeSH terms
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Adult
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Alkaline Phosphatase / analysis
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Alveolar Bone Loss / diagnostic imaging
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Alveolar Bone Loss / surgery
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Bone Regeneration* / physiology
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CD4 Antigens / analysis
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CD4-Positive T-Lymphocytes / pathology
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Coloring Agents
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Fibroblasts / pathology
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Guided Tissue Regeneration, Periodontal / instrumentation*
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Humans
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Immunoenzyme Techniques
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Immunohistochemistry
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Interferon-gamma / analysis
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Interleukin-4 / analysis*
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Leukocytes, Mononuclear / pathology
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Male
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Membranes, Artificial*
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Middle Aged
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Osteoblasts / pathology
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Osteopontin
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Periapical Diseases / diagnostic imaging
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Periapical Diseases / surgery
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Phosphoproteins / analysis
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Radiography
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Sialoglycoproteins / analysis
Substances
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CD4 Antigens
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Coloring Agents
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Membranes, Artificial
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Phosphoproteins
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SPP1 protein, human
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Sialoglycoproteins
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Osteopontin
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Interleukin-4
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Interferon-gamma
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Alkaline Phosphatase