Abstract
Retinoblastoma gene (Rb) defects occur frequently in human tumors. Studies of Rb-defective human tumor cell lines and Rb-/- murine embryonic fibroblasts demonstrate that Rb is required for interferon-gamma (IFN-gamma) induced major histocompatibility complex (MHC) class II expression. MHC class II expressing tumors generate anti-tumor immune responses associated with tumor-specific infiltrating lymphocytes. The role of Rb in IFN-gamma induced MHC class II expression on an endogenous tumor was examined by immunohistochemical staining for IAbeta and Rb on tissues from Rb+/- mice. MHC class II IAbeta is not induced by IFN-gamma in Rb-deficient neoplastic cells, but remains inducible in related normal tissue.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Female
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Gene Expression Regulation
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Genes, MHC Class II
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Genes, Retinoblastoma / genetics*
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Histocompatibility Antigens Class II / biosynthesis*
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Histocompatibility Antigens Class II / genetics
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Hyperplasia / immunology
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Hyperplasia / metabolism
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Immunohistochemistry
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Interferon-gamma / pharmacology
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Precancerous Conditions / genetics*
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Precancerous Conditions / immunology
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Precancerous Conditions / metabolism
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Retinoblastoma Protein / deficiency*
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Retinoblastoma Protein / physiology
Substances
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Histocompatibility Antigens Class II
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Retinoblastoma Protein
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Interferon-gamma