Leukocyte scintigraphy is a diagnostic method that allows visualisation of pathological accumulation of leukocytes. Some important indications are reviewed with special emphasis on the diagnostic value and possible limitations. Leukocyte scintigraphy is valuable for diagnosing osteomyelitis in bones with a pre-existing pathology, but probably not in chronic or vertebral osteomyelitis. The test is also valuable for detection of intra-abdominal abscess and vascular graft infection. Fever of unknown origin represents a diagnostic challenge. Although the sensitivity of leukocyte scintigraphy is only modest in this context, it seems to be a valuable addition to other tests performed in these patients.