First cyclotide from Hybanthus (Violaceae)

Phytochemistry. 2001 Sep;58(1):47-51. doi: 10.1016/s0031-9422(01)00173-x.

Abstract

Hypa A, a novel macrocyclic polypeptide containing 30 amino acid residues, has been isolated from the n-butanol extract of the Argentine plant Hybanthus parviflorus. The sequence, cyclo-(SCVYIPCTITALLGCSCKNKVCYNGIPCAE), was determined by automated Edman degradation, quantitative amino acid analysis and nanospray MS/MS(2). Three intramolecular disulfide bridges stabilize the cyclic peptide backbone of hypa A. Using these structural features to classify the peptide as a cyclotide, we extended the distribution of that substance class to a new genus, and now propose a uniform nomenclature for cyclotides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Argentina
  • Disulfides / chemistry
  • Drug Stability
  • Mass Spectrometry / methods
  • Molecular Sequence Data
  • Peptides, Cyclic / chemistry*
  • Peptides, Cyclic / isolation & purification
  • Rosaceae / chemistry*
  • Terminology as Topic

Substances

  • Disulfides
  • Peptides, Cyclic