Abstract
Screening of a Plasmodium falciparum genomic expression library for antigens expressed at the pre-erythrocytic stages resulted in the isolation of a recombinant phage (DG249) whose insert corresponded to regions II and III of a 175-kDa erythrocyte-binding antigen (EBA-175). EBA-175 is a parasite ligand implicated in red blood cell invasion. Reverse-transcriptase polymerase chain reaction, indirect immunofluorescent antibody test, and Western blot analysis confirmed that EBA-175 is expressed not only in blood-stage parasites but also in infected hepatocytes and on the sporozoite surface. The presence of EBA-175 on pre-erythrocytic parasites enhances the vaccine potential of this antigen by adding another target to the immune responses elicited by immunization.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Protozoan / blood
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Antibodies, Protozoan / immunology
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Antigens, Protozoan / genetics*
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Antigens, Protozoan / isolation & purification
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Blotting, Western
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Carrier Proteins / genetics*
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Carrier Proteins / isolation & purification
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Fluorescent Antibody Technique, Indirect
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Genomic Library
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Humans
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Liver / parasitology*
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Malaria, Falciparum / blood
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Malaria, Falciparum / parasitology
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Pan troglodytes / parasitology
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Plasmodium falciparum / growth & development
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Plasmodium falciparum / immunology*
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Protozoan Proteins / genetics*
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Protozoan Proteins / isolation & purification
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RNA, Messenger / analysis
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RNA, Protozoan / genetics
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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Antibodies, Protozoan
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Antigens, Protozoan
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Carrier Proteins
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Protozoan Proteins
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RNA, Messenger
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RNA, Protozoan
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erythrocyte-binding antigen 175, Plasmodium