Attempts to measure sensitiser photophysics in opaque tissue

J Photochem Photobiol B. 2001 Aug 30;61(3):129-36. doi: 10.1016/s1011-1344(01)00176-2.

Abstract

Diffuse reflectance laser flash photolysis has been used in an attempt to measure transient triplet spectra of the sensitisers, disulphonated aluminium phthalocyanine and porphyrin IX (derived from 5-amino laevulinic acid), in opaque tissue and models. The latter consisted of tissue phantoms; the former included rat liver and red blood cells. In all cases, triplet-triplet absorption spectra with relatively poor signal-to-noise were obtained providing some encouragement in the application of this technique to in vivo studies on photosensitisers.

MeSH terms

  • Aminolevulinic Acid / administration & dosage
  • Aminolevulinic Acid / metabolism
  • Animals
  • Erythrocytes / drug effects
  • Erythrocytes / metabolism*
  • Indoles / administration & dosage
  • Indoles / chemistry
  • Indoles / metabolism*
  • Liver / drug effects
  • Liver / metabolism*
  • Male
  • Molecular Structure
  • Organometallic Compounds / administration & dosage
  • Organometallic Compounds / chemistry
  • Organometallic Compounds / metabolism*
  • Photolysis
  • Photosensitizing Agents / administration & dosage
  • Photosensitizing Agents / chemistry
  • Photosensitizing Agents / metabolism*
  • Rats
  • Rats, Wistar

Substances

  • Indoles
  • Organometallic Compounds
  • Photosensitizing Agents
  • aluminum phthalocyanine disulfonate
  • Aminolevulinic Acid