Abstract
Environmental iron concentrations coordinately regulate transcription of genes involved in iron acquisition and virulence via the ferric uptake regulation (fur) system. We identified and sequenced the fur gene and flanking regions of three Bartonella species. The most notable difference between Bartonella Fur and other Fur proteins was a substantially higher predicted isoelectric point. No promoter activity or Fur autoregulation was detected using a gfp reporter gene fused to the 204 nucleotides immediately upstream of the Bartonella fur gene. Bartonella henselae fur gene expression complemented a Vibrio cholerae fur mutant.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins* / chemistry
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Bacterial Proteins* / genetics
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Bacterial Proteins* / metabolism
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Bartonella / classification
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Bartonella / genetics*
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Bartonella / metabolism*
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Blotting, Southern
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Gene Expression Regulation, Bacterial
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Genetic Complementation Test
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Humans
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Iron / metabolism
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Molecular Sequence Data
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Repressor Proteins* / chemistry
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Repressor Proteins* / genetics
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Repressor Proteins* / metabolism
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Sequence Analysis, DNA
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Vibrio cholerae / genetics
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Vibrio cholerae / metabolism
Substances
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Bacterial Proteins
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Repressor Proteins
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ferric uptake regulating proteins, bacterial
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Iron
Associated data
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GENBANK/AF388196
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GENBANK/AF388197
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GENBANK/AF388198