In vitro display technologies: novel developments and applications

Curr Opin Biotechnol. 2001 Aug;12(4):400-5. doi: 10.1016/s0958-1669(00)00234-2.

Abstract

In vitro display techniques are powerful tools to select polypeptide binders against various target molecules. Novel applications include maturation of protein affinity and stability, selection for enzymatic activity, and the display of cDNA and random polypeptide libraries. Taken together, these display techniques have great potential for biotechnological, medical and proteomic applications.

Publication types

  • Review

MeSH terms

  • Binding Sites / physiology
  • DNA, Complementary / genetics*
  • DNA, Complementary / metabolism
  • Directed Molecular Evolution / methods
  • Drug Evaluation, Preclinical / methods
  • Enzyme Stability / physiology
  • Gene Library*
  • Mutagenesis / genetics
  • Peptide Library
  • Proteins / genetics*
  • Proteins / metabolism
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism
  • Ribosomes / genetics*
  • Ribosomes / metabolism

Substances

  • DNA, Complementary
  • Peptide Library
  • Proteins
  • RNA, Messenger