Abstract
The ability of two glycoproteins of human milk, lactoferrin and free secretory component, to bind to Escherichia coli colonization factors (CFAs) was investigated using immunocytochemistry assays of enriched fimbrial extracts. The results revealed that lactoferrin binds to fimbrial CFA I adhesin but not to CFA II adhesin (CS1 and CS3), while free secretory component interacts with both CFA I and CFA II adhesins. Our data indicate that lactoferrin and free secretory component, which are very abundant proteins of human milk, could play an important role against infant enteric disease by blocking bacterial adhesion.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Adhesion / drug effects
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Bacterial Proteins / isolation & purification
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Bacterial Proteins / metabolism
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Escherichia coli / metabolism*
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Fimbriae Proteins*
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Glycoproteins / metabolism
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Humans
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Immunoblotting
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Lactoferrin / metabolism*
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Lactoferrin / pharmacology
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Microscopy, Immunoelectron
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Milk Proteins / metabolism*
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Milk, Human / chemistry
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Milk, Human / metabolism
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Protein Binding
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Secretory Component / metabolism*
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Secretory Component / pharmacology
Substances
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Bacterial Proteins
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Glycoproteins
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Milk Proteins
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Secretory Component
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colonization factor antigens
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Fimbriae Proteins
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Lactoferrin