Glyceraldehyde-3-phosphate dehydrogenase is responsible for intranuclear localization of some oligonucleotides

V Tomasi; P P Laktionov; A V Bryksin; N V Volod'ko; C Griffoni; E Y Rykova; E Spisni; R Kraft; V V Vlassov

doi:10.1081/NCN-100002447

Glyceraldehyde-3-phosphate dehydrogenase is responsible for intranuclear localization of some oligonucleotides

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):863-7. doi: 10.1081/NCN-100002447.

Authors

V Tomasi¹, P P Laktionov, A V Bryksin, N V Volod'ko, C Griffoni, E Y Rykova, E Spisni, R Kraft, V V Vlassov

Affiliation

¹ Laboratory of Nucleic Acids Biochemistry, Novosibirsk Institute of Bioorganic Chemistry, 8 Lavrentiev Ave., Novosibirsk, 630090, Russia.

PMID: 11563133
DOI: 10.1081/NCN-100002447

Abstract

Nuclear accumulation of ODNs has been associated with their binding to a series of nuclear proteins. These interactions could be responsible for the sequence-independent effects of ODNs as well as for their sequence-specific interactions and their intracellular distribution. Investigation of interaction of ODNs with these proteins may shed light on the mechanisms of cellular uptake and nuclear accumulation of oligonucleotides.

MeSH terms

Binding, Competitive
Cell Nucleus / metabolism
Endothelium / cytology
Endothelium / enzymology
Endothelium / metabolism
Glyceraldehyde-3-Phosphate Dehydrogenases / antagonists & inhibitors
Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism*
HeLa Cells
Humans
Monocytes / enzymology
Monocytes / metabolism
Oligonucleotides / pharmacokinetics*
Oligonucleotides / pharmacology

Substances

Oligonucleotides
Glyceraldehyde-3-Phosphate Dehydrogenases