Step-by-step control by MALDI-TOF MS of an oligonucleotide synthesis on standard CPG solid-support

A Meyer; F Debart; J L Imbach; J J Vasseur

doi:10.1081/NCN-100002466

Step-by-step control by MALDI-TOF MS of an oligonucleotide synthesis on standard CPG solid-support

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):951-4. doi: 10.1081/NCN-100002466.

Authors

A Meyer¹, F Debart, J L Imbach, J J Vasseur

Affiliation

¹ Lab. de Chimie Organique Biomoléculaire de Synthèse, UMR 5625 CNRS-UM2, Université Montpellier II, Place E. Bataillon, 34095, Montpellier, France.

PMID: 11563152
DOI: 10.1081/NCN-100002466

Abstract

MALDI-TOF mass spectrometry was used to monitor DNA solid-phase synthesis on long-chain alkylamine controlled-pore glass (LCAA-CPG) with a standard succinyl linker between the solid support and the growing oligonucleotide.

MeSH terms

Glass / chemistry
Oligonucleotides / chemical synthesis*
Organophosphorus Compounds / chemistry
Sequence Analysis, DNA / methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Oligonucleotides
Organophosphorus Compounds
phosphoramidite