Serotype-specific detection of coxsackievirus A16 in clinical specimens by reverse transcription-nested PCR

J Clin Microbiol. 2001 Oct;39(10):3690-2. doi: 10.1128/JCM.39.10.3690-3692.2001.

Abstract

We describe the development of a coxsackievirus A16 (CVA16) serotype-specific PCR which correctly differentiated between CVA16 and other enterovirus serotypes of both laboratory isolates and clinical specimens. The assay will be useful for monitoring CVA16 outbreaks and studying the disease association, epidemiology, and evolution of this common enterovirus serotype.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsid / genetics
  • Enterovirus / classification*
  • Enterovirus / genetics
  • Enterovirus / isolation & purification*
  • Enterovirus Infections / virology*
  • Humans
  • RNA, Viral
  • Reverse Transcriptase Polymerase Chain Reaction*
  • Serotyping
  • Species Specificity

Substances

  • RNA, Viral