Abstract
The detection of PBP 2a by the MRSA-Screen latex agglutination test with 201 clinical coagulase-negative staphylococci had an initial sensitivity of 98% and a high degree of specificity for Staphylococcus epidermidis strains compared to PCR for mecA. Determination of oxacillin MICs evaluated according to the new breakpoint (0.5 microg/ml) of the National Committee for Clinical Laboratory Standards exhibited an extremely low specificity for this population.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins*
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Carrier Proteins / metabolism*
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Coagulase / metabolism*
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Hexosyltransferases*
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Humans
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Latex Fixation Tests / methods*
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Methicillin Resistance* / genetics
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Microbial Sensitivity Tests / methods
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Muramoylpentapeptide Carboxypeptidase / metabolism*
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Oxacillin / pharmacology
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Penicillin-Binding Proteins
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Penicillins / pharmacology
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Peptidyl Transferases*
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Polymerase Chain Reaction
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Sensitivity and Specificity
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Staphylococcus / drug effects*
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Staphylococcus / enzymology
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Staphylococcus / genetics
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Time Factors
Substances
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Bacterial Proteins
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Carrier Proteins
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Coagulase
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Penicillin-Binding Proteins
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Penicillins
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Peptidyl Transferases
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Hexosyltransferases
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Muramoylpentapeptide Carboxypeptidase
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Oxacillin