[Basic study about the development of the hybrid-artificial liver support system using human hepatoma cell lines (Hep G2, Huh 7): effects on liver functions by extracellular matrix (type I collagen) in monolayer culture]

Fukuoka Igaku Zasshi. 2001 Aug;92(8):299-305.
[Article in Japanese]

Abstract

The risk of xenozoonosis infections poses the greatest obstacles against the clinical application of hybrid-artificial liver support system (HALSS). To resolve this issue, we used human hepatoma cell lines (Hep G2, Huh 7) in a type I collagen-coated monolayer culture system, and analyzed liver specific functions such as ammonia removal and albumin synthesis capacity. Ammonia removal activity (nmol/10(6) nuclei/hour) and albumin synthesis activity (microgram/10(6) nuclei/day) were upregulated in both Hep G2 and Huh 7 by type I collagen-coated monolayer culture. In particular, Hep G2 cultured in type I collagen-coated monolayer demonstrated relatively high ammonia removal and albumin synthesis capacity. These results indicate the possibility of the application of human hepatocytes to HALSS.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Albumins / biosynthesis
  • Ammonia / metabolism
  • Carcinoma, Hepatocellular*
  • Collagen Type I / pharmacology*
  • Extracellular Matrix*
  • Humans
  • Liver / cytology*
  • Liver / physiology*
  • Liver Neoplasms*
  • Liver, Artificial*
  • Tumor Cells, Cultured
  • Up-Regulation

Substances

  • Albumins
  • Collagen Type I
  • Ammonia