A murine dopamine neuron-specific cDNA library and microarray: increased COX1 expression during methamphetamine neurotoxicity

Neurobiol Dis. 2001 Oct;8(5):822-33. doi: 10.1006/nbdi.2001.0423.

Abstract

Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter-lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greater than twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantia nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3,4-Dihydroxyphenylacetic Acid / analysis
  • Animals
  • DNA, Complementary / genetics
  • Dopamine / analysis*
  • Dopamine Uptake Inhibitors / toxicity*
  • Electron Transport Complex IV / biosynthesis*
  • Electron Transport Complex IV / genetics
  • Enzyme Induction
  • Female
  • Gene Expression Profiling*
  • Gene Library*
  • Genes, Synthetic
  • Lac Operon
  • Male
  • Mesencephalon / cytology
  • Mesencephalon / embryology
  • Methamphetamine / toxicity*
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Nerve Tissue Proteins / biosynthesis*
  • Nerve Tissue Proteins / genetics
  • Neurons / metabolism*
  • Oligonucleotide Array Sequence Analysis*
  • Promoter Regions, Genetic
  • Rats
  • Transcription, Genetic
  • Tyrosine 3-Monooxygenase / genetics

Substances

  • DNA, Complementary
  • Dopamine Uptake Inhibitors
  • Nerve Tissue Proteins
  • 3,4-Dihydroxyphenylacetic Acid
  • Methamphetamine
  • Tyrosine 3-Monooxygenase
  • Electron Transport Complex IV
  • Dopamine

Associated data

  • GENBANK/BE824471
  • GENBANK/BE824475
  • GENBANK/BE824488
  • GENBANK/BE824509
  • GENBANK/BE824514
  • GENBANK/BE824515
  • GENBANK/BE824534
  • GENBANK/BE824540
  • GENBANK/BE824543
  • GENBANK/BE824549
  • GENBANK/BE824580
  • GENBANK/BE824608
  • GENBANK/BE824623
  • GENBANK/BE824737
  • GENBANK/BE824847
  • GENBANK/BE824874
  • GENBANK/BE824891
  • GENBANK/BE824897
  • GENBANK/BE824908
  • GENBANK/BE824917
  • GENBANK/BE825801