Simultaneous determination of six triazolic pesticide residues in apple and pear pulps by liquid chromatography with ultraviolet diode array detection

J AOAC Int. 2001 Sep-Oct;84(5):1543-50.

Abstract

A method is described for the simultaneous determination of diclobutrazol, flusilazole, flutriafol, hexaconazole, paclobutrazol, and tetraconazole in apple and pear pulps used in baby food at a limit of 0.01 mg/kg. Apple and pear pulp samples are subjected to selective solid-phase microdispersion (SPMD) with SPE-ED Matrix-38 and acetone-cyclohexane, and the extracts are cleaned up on a Florisil cartridge with hexane-cyclohexane-acetone. The extracts are then analyzed by liquid chromatography with ultraviolet detection, using an octadecylsilane column with a gradient-programmed acetonitrile-water mobile phase. Recoveries were determined by spiking apple and pear pulps with the 6 pesticides under investigation at 0.1, 0.05, 0.03, and 0.01 mg/kg. Six determinations were performed at each level for each pesticide. Recoveries were > or = 70% at the 0.01 mg/kg level.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calibration
  • Chromatography, Liquid
  • Fruit / chemistry*
  • Humans
  • Indicators and Reagents
  • Infant
  • Infant Food / analysis*
  • Malus / chemistry*
  • Pesticide Residues / analysis*
  • Reference Standards
  • Solvents
  • Spectrophotometry, Ultraviolet
  • Triazoles / analysis*

Substances

  • Indicators and Reagents
  • Pesticide Residues
  • Solvents
  • Triazoles