Efficient FLP recombination in mouse ES cells and oocytes

Genesis. 2001 Sep;31(1):6-10. doi: 10.1002/gene.1076.

Abstract

We report an improved vector, pCAGGS-FLPe, for transient expression of the enhanced FLP recombinase in mouse ES cells and oocytes. In standard transfection experiments, about 6% of total ES colonies showed FLP recombination, albeit with mosaicism within each colony. After microinjection of pCAGGS-FLPe into oocytes, about one-third of heterozygotic mice born showed complete FLP recombination. Thus pCAGGS-FLPe presents two practical options for removal of FRT cassettes in mice.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA Nucleotidyltransferases / genetics*
  • DNA Nucleotidyltransferases / metabolism
  • DNA Primers / chemistry
  • Embryo, Mammalian / enzymology*
  • Female
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Developmental
  • Genetic Engineering / methods
  • Genetic Vectors
  • Male
  • Mice
  • Mice, Inbred Strains
  • Mice, Transgenic
  • Molecular Sequence Data
  • Oocytes / enzymology*
  • Polymerase Chain Reaction
  • Transfection

Substances

  • DNA Primers
  • Fungal Proteins
  • DNA Nucleotidyltransferases
  • FLP recombinase