Comprehensive analysis of a large genomic sequence at the putative B-cell chronic lymphocytic leukaemia (B-CLL) tumour suppresser gene locus

Mutat Res. 2001 Dec;458(3-4):55-70. doi: 10.1016/s0027-5107(01)00219-6.

Abstract

In many haematological diseases, and more particularly in B-cell chronic lymphocytic leukaemia (B-CLL), the existence of a tumour suppressor gene located within the frequently deleted region 13q14.3, has been put forward. A wide candidate region spanning from marker D13S273 to D13S25 has been proposed and an extensive physical map has been constructed by several teams. In this study, we sequenced a minimal core deleted region that we have previously defined and annotated it with flanking available public sequences. Our analysis shows that this region is gene-poor. Furthermore, our work allowed us to identify new alternative transcripts, spanning core regions, of the previously defined candidate genes DLEU1 and DLEU2. Since their putative involvement in B-CLL was controversial, our present study provide support for reconsidering the DLEU1 and DLEU2 genes as B-CLL candidate genes, with a new definition of their organisation and context.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing / genetics
  • B-Lymphocytes / metabolism*
  • Base Sequence
  • Chromosome Mapping
  • Databases, Nucleic Acid
  • Exons / genetics
  • Expressed Sequence Tags
  • Genes, Tumor Suppressor*
  • Humans
  • Leukemia, Lymphocytic, Chronic, B-Cell / genetics*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Repetitive Sequences, Nucleic Acid / genetics
  • Sequence Analysis, DNA
  • Sequence Deletion / genetics*