Isolation of X and Y chromosome-specific DNA markers from a liverwort, Marchantia polymorpha, by representational difference analysis

M Fujisawa; K Hayashi; T Nishio; T Bando; S Okada; K T Yamato; H Fukuzawa; K Ohyama

doi:10.1093/genetics/159.3.981

Isolation of X and Y chromosome-specific DNA markers from a liverwort, Marchantia polymorpha, by representational difference analysis

Genetics. 2001 Nov;159(3):981-5. doi: 10.1093/genetics/159.3.981.

Authors

M Fujisawa¹, K Hayashi, T Nishio, T Bando, S Okada, K T Yamato, H Fukuzawa, K Ohyama

Affiliation

¹ Laboratory of Plant Molecular Biology, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.

Abstract

The liverwort Marchantia polymorpha has X and Y chromosomes in the respective female and male haploids. Here we report the successful exploitation of representational difference analyses to isolate DNA markers for the sex chromosomes. Two female-specific and six male-specific DNA fragments were genetically confirmed to originate from the X and Y chromosomes, respectively.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosomes / genetics*
Crosses, Genetic
DNA Primers / genetics
Gene Expression Profiling / methods
Genetic Linkage
Genetic Markers*
Genome, Plant
Models, Genetic
Nucleic Acid Hybridization
Plants / genetics*
Polymerase Chain Reaction

Substances

DNA Primers
Genetic Markers