Abstract
A fluorescent analog of epidermal mitosis-inhibiting pentapeptide (pGlu-Glu-Asp-Ser-Gly) was synthesized by reacting tetramethylrhodamine isothiocyanate with ring-opened epidermal mitosis-inhibiting pentapeptide. The ring-opening reaction of the pyrrolidone moiety was performed with mild acidic hydrolysis and the product purified by reversed-phase high-performance liquid chromatography. Tetramethylrhodaminethiocarbamoyl-(Glu(1))-epidermal mitosis-inhibiting pentapeptide was purified by chromatography on Sephadex G-25 and reversed-phase high-performance liquid chromatography. After characterization by amino acid analysis, the analog was incubated in presence of A431 cell line to visualize the cellular localization of the epidermal mitosis-inhibiting pentapeptide. The data gave negative results.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Adhesion / drug effects
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Chromatography, Gel
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Chromatography, High Pressure Liquid
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Chromatography, Thin Layer
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Fluorescent Dyes / metabolism
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Glutamic Acid / analogs & derivatives
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Glutamic Acid / chemical synthesis*
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Glutamic Acid / isolation & purification
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Glutamic Acid / pharmacology*
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Humans
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Microscopy, Confocal
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Mitosis / drug effects*
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Peptides / chemical synthesis*
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Peptides / chemistry
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Peptides / isolation & purification
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Peptides / pharmacology*
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Rhodamines / chemical synthesis*
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Rhodamines / isolation & purification
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Rhodamines / metabolism
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Rhodamines / pharmacology*
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Spectrophotometry, Ultraviolet
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Tumor Cells, Cultured
Substances
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Fluorescent Dyes
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Peptides
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Rhodamines
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tetramethylrhodaminethiocarbamoyl-(Glu(1))
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Glutamic Acid
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tetramethylrhodamine isothiocyanate