Abstract
6-Nitrocatecholamines were recently described as novel neuronal nitric oxide synthase inhibitors competing with both L-arginine and tetrahydrobiopterin (BH(4)). We report now that simple nitrocatechols are also competitive inhibitors, lacking however BH(4)-antagonizing properties. It is argued that 6-nitrocatecholamines interact with the L-arginine- and BH(4)-binding sites through the nitrocatechol and aminoethyl moieties, respectively.
MeSH terms
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Antioxidants / chemistry
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Antioxidants / pharmacology
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Arginine / pharmacology
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Binding Sites
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Biopterins / analogs & derivatives*
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Biopterins / chemistry
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Catecholamines / chemistry*
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Catecholamines / pharmacology
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Catechols / chemistry*
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Catechols / pharmacology
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Drug Evaluation, Preclinical
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Humans
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Inhibitory Concentration 50
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Kinetics
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Nitric Oxide Synthase / antagonists & inhibitors*
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Nitric Oxide Synthase / metabolism
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Nitric Oxide Synthase Type I
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Nitro Compounds / chemistry*
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Nitro Compounds / pharmacology
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Protein Isoforms / antagonists & inhibitors
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Protein Isoforms / metabolism
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Structure-Activity Relationship
Substances
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Antioxidants
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Catecholamines
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Catechols
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Nitro Compounds
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Protein Isoforms
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Biopterins
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Arginine
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NOS1 protein, human
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Nitric Oxide Synthase
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Nitric Oxide Synthase Type I
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sapropterin