Abstract
Heteromultimeric cyclic nucleotide-gated (CNG) channels play a central role in the transduction of odorant signals and subsequent adaptation. The contributions of individual subunits to native channel function in olfactory receptor neurons remain unclear. Here, we show that the targeted deletion of the mouse CNGA4 gene, which encodes a modulatory CNG subunit, results in a defect in odorant-dependent adaptation. Channels in excised membrane patches from the CNGA4 null mouse exhibited slower Ca2+-calmodulin-mediated channel desensitization. Thus, the CNGA4 subunit accelerates the Ca2+-mediated negative feedback in olfactory signaling and allows rapid adaptation in this sensory system.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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1-Methyl-3-isobutylxanthine / pharmacology
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Adaptation, Physiological*
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Animals
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Calcium / metabolism*
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Calcium Signaling
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Calmodulin / metabolism*
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Cyclic AMP / metabolism
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Cyclic Nucleotide-Gated Cation Channels
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Cyclohexanols / pharmacology
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Electrophysiology
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Eucalyptol
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Gene Targeting
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Ion Channel Gating
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Ion Channels / genetics*
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Ion Channels / physiology*
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Kinetics
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Mice
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Mice, Inbred C57BL
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Monoterpenes*
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Odorants*
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Olfactory Bulb / physiology
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Olfactory Mucosa / physiology
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Olfactory Receptor Neurons / metabolism
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Olfactory Receptor Neurons / physiology*
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Protein Subunits
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Terpenes / pharmacology
Substances
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Calmodulin
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Cyclic Nucleotide-Gated Cation Channels
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Cyclohexanols
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Ion Channels
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Monoterpenes
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Protein Subunits
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Terpenes
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Cyclic AMP
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Eucalyptol
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Calcium
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1-Methyl-3-isobutylxanthine