Translocation portals for the substrates and products of a viral transcription complex: the bluetongue virus core

EMBO J. 2001 Dec 17;20(24):7229-39. doi: 10.1093/emboj/20.24.7229.

Abstract

The bluetongue virus core is a molecular machine that simultaneously and repeatedly transcribes mRNA from 10 segments of viral double-stranded RNA, packaged in a liquid crystalline array. To determine how the logistical problems of transcription within a sealed shell are solved, core crystals were soaked with various ligands and analysed by X-ray crystallography. Mg(2+) ions produce a slight expansion of the capsid around the 5-fold axes. Oligonucleotide soaks demonstrate that the 5-fold pore, opened up by this expansion, is the exit site for mRNA, whilst nucleotide soaks pinpoint a separate binding site that appears to be a selective channel for the entry and exit of substrates and by-products. Finally, nucleotides also bind to the outer core layer, providing a substrate sink.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Binding Sites
  • Bluetongue virus / genetics
  • Bluetongue virus / physiology*
  • Calcium / metabolism
  • Crystallography, X-Ray
  • Magnesium / metabolism
  • Phosphates / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sulfates / metabolism
  • Transcription, Genetic*

Substances

  • Phosphates
  • RNA, Messenger
  • Sulfates
  • Magnesium
  • Calcium