Abstract
Sensitivity for the detection of mecA-positive coagulase-negative staphylococci (CNS) was evaluated for different breakpoints of resistance for oxacillin using three different susceptibility tests, either on Mueller-Hinton agar supplemented with 2% NaCl (MH-NaCl agar) or on paper disc method agar supplemented with 5% defibrinated blood (PDM-blood agar). The Etest, multipoint inoculation test and disc diffusion test showed comparable sensitivity (0.96, 0.96 and 0.95, respectively) using an oxacillin breakpoint of > or = 0.5 mg/L or < or = 17 mm for the disc test, after incubation at 35 degrees C for 24 h on MH-NaCl agar. The sensitivity decreased for breakpoints > or = 1 mg/L and when PDM-blood agar was used instead of MH-NaCl agar.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Bacterial Proteins*
-
Bacteriological Techniques / methods
-
Carrier Proteins / genetics*
-
Carrier Proteins / isolation & purification
-
Coagulase / genetics*
-
Coagulase / isolation & purification
-
Culture Media / pharmacology
-
Drug Resistance, Bacterial / genetics
-
Hexosyltransferases*
-
Humans
-
Microbial Sensitivity Tests / methods
-
Microbial Sensitivity Tests / statistics & numerical data
-
Muramoylpentapeptide Carboxypeptidase / genetics*
-
Muramoylpentapeptide Carboxypeptidase / isolation & purification
-
Oxacillin / pharmacology
-
Penicillin-Binding Proteins
-
Penicillins / pharmacology
-
Peptidyl Transferases*
-
Phenotype
-
Staphylococcus / drug effects
-
Staphylococcus / genetics*
-
Staphylococcus / isolation & purification
Substances
-
Bacterial Proteins
-
Carrier Proteins
-
Coagulase
-
Culture Media
-
Penicillin-Binding Proteins
-
Penicillins
-
Peptidyl Transferases
-
Hexosyltransferases
-
Muramoylpentapeptide Carboxypeptidase
-
Oxacillin