Role of the Nha1 antiporter in regulating K(+) influx in Saccharomyces cerevisiae

Yeast. 2002 Jan 15;19(1):9-15. doi: 10.1002/yea.799.

Abstract

NHA1 encodes a K(+) (Na(+))/H(+) antiporter in the plasma membrane of Saccharomyces cerevisiae. We report that cells expressing the NHA1 gene contained less K(+) than the mutant lacking the gene when grown without K(+) limitation. They also grew better at low K(+) and showed higher affinity of transport than the nha1 strain. In agreement with the function of an electroneutral cation/H(+) antiporter, the effect was only observed at acidic pH. The improved growth and transport depended on the presence of Trk1p (the main K(+) influx system) and did not require the product of TRK2. We propose that Nha1p regulates the potassium content of the cell and, as a consequence, can affect the activity of the main K(+) influx system (Trk1p).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Transport
  • Carrier Proteins / genetics
  • Carrier Proteins / physiology
  • Cation Transport Proteins*
  • Culture Media
  • Hydrogen-Ion Concentration
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Membrane Proteins / physiology
  • Mutation
  • Potassium / metabolism*
  • Rubidium / metabolism
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • Sodium-Hydrogen Exchangers / genetics
  • Sodium-Hydrogen Exchangers / metabolism*

Substances

  • Carrier Proteins
  • Cation Transport Proteins
  • Culture Media
  • Membrane Proteins
  • NHA1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Sodium-Hydrogen Exchangers
  • TRK1 protein, S cerevisiae
  • Rubidium
  • Potassium