This paper present the concentration profiles of the organochlorine pesticides in a sediment core from Macao Estuary, Pearl River, China. Concentrations of organochlorine pesticides were determined by GC/ ECD and GC/MSD, and the core was dated by 210Pb method in a content-activity model. The results show that the concentrations of HCHs and DDTs in the core ranged from 0.48-26.3 and 1.92-39.1 microg/kg, respectively. Concentration of DDTs in the research area was above the Effects Range-Low value (ERL) for Marine and Estuarine sediments. The vertical distributing of organochlorine pesticides in the core was influenced by the water flux of Pearl River and the pollutants are mainly attributed to the runoff of local agricultural soils.