Tocopherols and carotenoids are naturally occurring lipophilic micronutrients, suggested to play a role in the prevention of several degenerative diseases. Thus, methods for the quantification of these nutrients in human samples have been developed during recent years. Blood and tissue levels of tocopherols and carotenoids are influenced by a variety of parameters related to disease, age, diet and lifestyle. This review summarizes general aspects of chromatographic analysis of tocopherols and carotenoids in human samples and deals with information on the outcome of human studies, in which such measurements were applied.