Abstract
Isolates of Clostridium difficile from different hospital wards at the University Hospital of Szeged in Hungary were typed by PCR amplification of rRNA intergenic spacer regions (PCR ribotyping). A total of 15 different ribotypes was detected among the 65 isolates tested. The predominant type, PCR ribotype 087, accounted for 39% of all isolates, in contrast with an international typing study where ribotype 001 was the most common. Two non-toxigenic C. difficile strains were found to exhibit the same pattern, which was distinct from those of all the ribotypes described previously, suggesting that this is a new type.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Proteins*
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Bacterial Toxins / analysis*
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Bacterial Toxins / toxicity
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Chlorocebus aethiops
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Clostridioides difficile / classification*
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Clostridioides difficile / genetics
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Clostridioides difficile / isolation & purification
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Cross Infection
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Enterocolitis, Pseudomembranous / epidemiology
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Enterocolitis, Pseudomembranous / microbiology*
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Feces / microbiology
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Gene Amplification
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Humans
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Hungary / epidemiology
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Infant, Newborn
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Polymerase Chain Reaction / methods
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RNA, Ribosomal / genetics*
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Ribotyping
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Vero Cells
Substances
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Bacterial Proteins
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Bacterial Toxins
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RNA, Ribosomal
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toxB protein, Clostridium difficile