Two-stage polymerase chain reaction protocol allowing introduction of multiple mutations, deletions, and insertions, using QuikChange site-directed mutagenesis

Methods Mol Biol. 2002:182:37-43. doi: 10.1385/1-59259-194-9:037.
No abstract available

MeSH terms

  • Base Sequence
  • DNA Primers / chemical synthesis
  • Electrophoresis, Agar Gel / methods
  • Genetic Engineering / methods
  • Indicators and Reagents
  • Molecular Sequence Data
  • Mutagenesis
  • Mutagenesis, Insertional*
  • Mutagenesis, Site-Directed*
  • Polymerase Chain Reaction / methods*
  • Sequence Deletion*

Substances

  • DNA Primers
  • Indicators and Reagents