Objective: To investigate the effects of E1A gene on proliferation and metastasis of human lung adenocarcinoma cells.
Methods: A mammalian E1A-expressing recombinant constructed in our laboratory, named pcDNA3-E1A, was introduced into human lung adenocarcinoma cell line(Anip 973) by lipofectamine. The cells resistant to G418 were selected. The characteristics of the E1A-expressing Anip 973(Anip 973-E1A) cells were studied in vitro and in vivo, including cell growth rate and doubling-time, colony-forming efficiency on soft agarose, tumorigenicity and metastasis.
Results: The growth rate of E1A-Anip 973 cells was diminished and their colony-forming efficiency was inhibited significantly. The tumorigenicity in nude mice of the E1A-expressing Anip 973 was markedly suppressed.
Conclusion: E1A suppresses the malignant phenotype of human lung adenocarcinoma cell line. This study provides experimental data for gene therapy of lung cancer with E1A.