Interaction between interleukin 1beta and endogenous neurokinin 1 receptor agonists in mediating plasma extravasation and neutrophil accumulation in the cutaneous microvasculature of the rat

Neurosci Lett. 2002 Jan 18;318(1):13-6. doi: 10.1016/s0304-3940(01)02468-5.

Abstract

Interleukin 1beta (IL-1beta) is a potent mediator of neutrophil accumulation. Antidromic stimulation of the rat saphenous nerve leads to neurogenic oedema formation mediated by endogenous tachykinins. Here, we have investigated links between IL-1beta and the tachykinin 1 (NK(1)) receptors in microvascular events in rat skin. Saphenous nerve-induced plasma extravasation was not modulated by skin pretreatment with IL-1beta (3 pmol/site intradermally). In addition, the long-lasting antidromic electrical stimulation did not induce significant neutrophil accumulation in naive rat skin. By comparison, the effect of IL-1beta-induced neutrophil accumulation was significantly potentiated by co-stimulation of the ipsilateral saphenous nerve; an effect prevented by an NK(1) receptor antagonist (SR140333, 480 nmol/kg, i.v.). We conclude that IL-1beta-induced neutrophil accumulation can be influenced in a pro-inflammatory manner by ongoing neurogenic inflammation, of relevance to the sensory nerve input that occurs during ongoing inflammatory disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Edema / metabolism
  • Electric Stimulation
  • Interleukin-1 / pharmacology*
  • Male
  • Microcirculation / physiology
  • Neuritis / metabolism
  • Neurokinin-1 Receptor Antagonists
  • Neutrophils / cytology*
  • Neutrophils / drug effects
  • Piperidines / pharmacology
  • Plasma / metabolism
  • Quinuclidines / pharmacology
  • Rats
  • Rats, Wistar
  • Receptors, Neurokinin-1 / agonists
  • Receptors, Neurokinin-1 / metabolism*
  • Sciatic Nerve / physiology
  • Sensory Receptor Cells / physiology
  • Skin / blood supply*
  • Skin / immunology
  • Skin / innervation*
  • Tachykinins / metabolism

Substances

  • Interleukin-1
  • Neurokinin-1 Receptor Antagonists
  • Piperidines
  • Quinuclidines
  • Receptors, Neurokinin-1
  • Tachykinins
  • SR 140333