Effects of parenterally administered ciprofloxacin in a murine model of pulmonary Pseudomonas aeruginosa infection mimicking ventilator-associated pneumonia

Chemotherapy. 2001 Dec;47(6):421-9. doi: 10.1159/000048553.

Abstract

Background and methods: We compared the bacteriological, pharmacological and histopathological effects of parenterally administered ciprofloxacin (CPFX) to those of imipenem/cilastatin (IMP/CS) and cefozopran (CZOP) in a murine model of mucoid Pseudomonas aeruginosa pneumonia mimicking ventilator-associated pneumonia.

Results: The minimum inhibitory concentrations (MICs) of CPFX, IMP and CZOP were 1.0, 1.0 and 4.0 mg/l, respectively. Treatment with CPFX resulted in a significant decrease in the number of viable bacteria [control, IMP/CS, CZOP and CPFX (mean +/- SEM): 5.02 +/- 0.20, 4.96 +/- 0.38, 5.44 +/- 0.13 and 3.27 +/- 0.02 log(10) colony-forming units lung, respectively]. Histopathological examination revealed that inflammatory changes in the CPFX-treated group were less marked than in other groups. Of the drugs analyzed, the pharmacokinetic parameters of area under the time-concentration curve (AUC)/MIC, AUC exceeding MIC and the time that lung concentrations of drug remained above the MIC were highest for CPFX.

Conclusion: Our results suggest that parenterally administered ciprofloxacin is effective in ventilator-associated P. aeruginosa pneumonia.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Anti-Infective Agents / administration & dosage
  • Anti-Infective Agents / pharmacokinetics
  • Anti-Infective Agents / pharmacology*
  • Area Under Curve
  • Cefozopran
  • Cephalosporins / administration & dosage
  • Cephalosporins / pharmacokinetics
  • Cephalosporins / pharmacology*
  • Cilastatin / administration & dosage
  • Cilastatin / pharmacokinetics
  • Cilastatin / pharmacology*
  • Ciprofloxacin / administration & dosage
  • Ciprofloxacin / pharmacokinetics
  • Ciprofloxacin / pharmacology*
  • Disease Models, Animal
  • Imipenem / administration & dosage
  • Imipenem / pharmacokinetics
  • Imipenem / pharmacology*
  • Infusions, Parenteral
  • Male
  • Mice
  • Pneumonia, Bacterial / drug therapy*
  • Pneumonia, Bacterial / veterinary
  • Protease Inhibitors / administration & dosage
  • Protease Inhibitors / pharmacokinetics
  • Protease Inhibitors / pharmacology*
  • Pseudomonas Infections / drug therapy*
  • Pseudomonas Infections / veterinary
  • Pseudomonas aeruginosa / pathogenicity*
  • Respiration, Artificial / adverse effects*
  • Thienamycins / administration & dosage
  • Thienamycins / pharmacokinetics
  • Thienamycins / pharmacology*

Substances

  • Anti-Infective Agents
  • Cephalosporins
  • Protease Inhibitors
  • Thienamycins
  • Cilastatin
  • Ciprofloxacin
  • Imipenem