Cutting edge: precursor frequency affects the helper dependence of cytotoxic T cells

J Immunol. 2002 Feb 1;168(3):977-80. doi: 10.4049/jimmunol.168.3.977.

Abstract

Generation of CTL immunity often depends on the availability of CD4 T cell help. In this report, we show that CTL responses induced by cross-priming can be converted from CD4-dependent to CD4-independent by increasing the frequency of CTL precursors. In the absence of CD4 T cells, high numbers of CTL precursors were able to expand in number and become effector CTL. The ability of high frequencies of CD8 T cells to override help was not due to their ability to signal CD40 via expression of CD154. These findings suggest that when precursor frequencies are high, priming of CD8 T cell responses may not require CD4 T cell help.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens / pharmacology
  • CD40 Ligand / biosynthesis
  • CD40 Ligand / physiology
  • Epitopes, T-Lymphocyte / administration & dosage
  • Epitopes, T-Lymphocyte / immunology
  • Histocompatibility Antigens Class II / genetics
  • Injections, Intravenous
  • Lymphocyte Activation / genetics
  • Lymphocyte Count
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Ovalbumin / administration & dosage
  • Ovalbumin / genetics
  • Ovalbumin / immunology
  • Spleen / cytology
  • Spleen / immunology
  • Spleen / transplantation
  • Stem Cells / cytology*
  • Stem Cells / immunology*
  • T-Lymphocytes, Cytotoxic / cytology*
  • T-Lymphocytes, Cytotoxic / immunology*
  • T-Lymphocytes, Cytotoxic / transplantation
  • T-Lymphocytes, Helper-Inducer / immunology*

Substances

  • Antigens
  • Epitopes, T-Lymphocyte
  • Histocompatibility Antigens Class II
  • CD40 Ligand
  • Ovalbumin