Establishment of a non-tumorigenic papillary thyroid cell line (FB-2) carrying the RET/PTC1 rearrangement

Int J Cancer. 2002 Feb 10;97(5):608-14. doi: 10.1002/ijc.10116.

Abstract

A novel human thyroid papillary carcinoma cell line (FB-2) has been established and characterized. FB-2 cells harbor the RET/PTC1 chimeric oncogene in which the RET kinase domain is fused to the H4 gene. FB-2 cells neither formed colonies in semisolid media nor induced tumors after heterotransplant into severe combined immunodeficient mice. However, HMGI(Y), HMGI-C and c-myc genes, which are associated to thyroid cell transformation, were abundantly expressed in FB-2 cells but not in normal thyroid cells. FB-2 cells only partially retained the differentiated thyroid phenotype. In fact, the PAX-8 gene, which codes for a transcriptional factor required for thyroid cell differentiation, was expressed, while thyroglobulin, TSH-receptor and thyroperoxidase genes were not. Moreover, FB-2 cells produced high levels of interleukin (IL)-6 and IL-8.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Carcinoma, Papillary / genetics*
  • Carcinoma, Papillary / metabolism
  • Carcinoma, Papillary / pathology
  • Cell Differentiation
  • Cell Division / drug effects
  • Cell Movement
  • DNA-Binding Proteins / biosynthesis
  • Female
  • HMGA1a Protein / biosynthesis
  • HMGA1a Protein / genetics
  • HMGA2 Protein / biosynthesis
  • HMGA2 Protein / genetics
  • Humans
  • Interleukin-6 / biosynthesis
  • Interleukin-6 / pharmacology
  • Interleukin-8 / biosynthesis
  • Karyotyping
  • Mice
  • Mice, SCID
  • Neoplasm Transplantation
  • Neoplasms, Experimental / genetics*
  • Neoplasms, Experimental / metabolism
  • Neoplasms, Experimental / pathology
  • Nuclear Proteins*
  • Oncogene Proteins, Fusion / genetics*
  • Oncogene Proteins, Fusion / metabolism
  • PAX8 Transcription Factor
  • Paired Box Transcription Factors
  • Phenotype
  • Protein-Tyrosine Kinases
  • Proto-Oncogene Proteins c-myc / biosynthesis
  • Proto-Oncogene Proteins c-myc / genetics
  • RNA, Messenger / biosynthesis
  • Thyroid Gland / cytology
  • Thyroid Gland / metabolism
  • Thyroid Neoplasms / genetics*
  • Thyroid Neoplasms / metabolism
  • Thyroid Neoplasms / pathology
  • Trans-Activators / biosynthesis
  • Tumor Cells, Cultured

Substances

  • DNA-Binding Proteins
  • HMGA2 Protein
  • Interleukin-6
  • Interleukin-8
  • Nuclear Proteins
  • Oncogene Proteins, Fusion
  • PAX8 Transcription Factor
  • PAX8 protein, human
  • Paired Box Transcription Factors
  • Pax8 protein, mouse
  • Proto-Oncogene Proteins c-myc
  • RNA, Messenger
  • Trans-Activators
  • HMGA1a Protein
  • Protein-Tyrosine Kinases
  • ret-PTC fusion oncoproteins, human