DNA microsatellite analysis for parentage control in Austrian pigs

Anim Biotechnol. 2001 Nov;12(2):141-4. doi: 10.1081/ABIO-100108340.

Abstract

We present an efficient parentage control for pigs based on ten polymorphic microsatellite markers analyzed in a single PCR reaction. Assuming one known parent ("paternity control"), combined exclusion probabilities (CEPs) ranged from 99.18% (Landrace), 99.74% (Piétrain) to 99.76% (Large White) for the most important Austrian breeds. Assuming a known parent-pair ("parentage control", e.g. a substituted offspring), the CEP of the 10-plex PCR increased to 99.97% (Landrace) and 99.99% (Piétrain and Large White). We developed an additional standby battery of 5 markers, which might be applied in those cases, where the CEP of the 10-plex PCR is not sufficient. Therefore an automated, cost and time reduced genotype analysis for pigs is available.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animal Husbandry
  • Animals
  • Automation
  • Female
  • Male
  • Microsatellite Repeats*
  • Pedigree
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Probability
  • Swine / genetics*