Meropenem stability to beta-lactamase hydrolysis and comparative in vitro activity against several beta-lactamase-producing Gram-negative strains

N Franceschini; B Segatore; M Perilli; S Vessillier; L Franchino; G Amicosante

doi:10.1093/jac/49.2.395

Meropenem stability to beta-lactamase hydrolysis and comparative in vitro activity against several beta-lactamase-producing Gram-negative strains

J Antimicrob Chemother. 2002 Feb;49(2):395-8. doi: 10.1093/jac/49.2.395.

Authors

N Franceschini¹, B Segatore, M Perilli, S Vessillier, L Franchino, G Amicosante

Affiliation

¹ Dipartimento di Scienze e Tecnologie Biomediche, Università dell'Aquila, Via Vetoio, 67100 L'Aquila, Italy.

PMID: 11815587
DOI: 10.1093/jac/49.2.395

Abstract

The interaction between meropenem and class A, B, C and D beta-lactamases was studied by a spectrophotometric method. Class A, C and D beta-lactamases were unable to confer in vitro resistance to carbapenems. Surprisingly, several class B metallo-beta-lactamases expressed in Escherichia coli failed to confer resistance when a conventional inoculum (105 cfu/mL) was used.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Enzyme Activation / drug effects
Gram-Negative Bacteria / drug effects
Gram-Negative Bacteria / enzymology*
Gram-Negative Bacteria / growth & development
Hydrolysis
Meropenem
Microbial Sensitivity Tests / statistics & numerical data
Thienamycins / pharmacokinetics*
Thienamycins / pharmacology
beta-Lactamases / biosynthesis
beta-Lactamases / classification
beta-Lactamases / metabolism*

Substances

Thienamycins
beta-Lactamases
Meropenem