Abstract
The E2F family of transcription factors plays a pivotal role in the regulation of cell proliferation in higher eukaryotes. We used DNA microarrays and cell lines containing either inducible E2F-1 or inducible E2F-3 to identify novel E2F target genes. Our data indicate that E2F up-regulates the expression of genes not previously described as E2F target genes. A number of these E2F-regulated genes are involved in DNA replication, DNA repair and mitosis. These results suggest that E2F affects cell cycle progression both at S phase and during mitosis. Furthermore, our findings indicate that E2F-dependent gene activation may contribute to the cellular response to DNA damage.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western
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Cell Cycle / genetics
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Cell Cycle Proteins*
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Cells, Cultured
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DNA Repair / genetics*
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DNA Replication / genetics*
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DNA-Binding Proteins*
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E2F Transcription Factors
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E2F1 Transcription Factor
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E2F3 Transcription Factor
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Fibroblasts
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Flow Cytometry
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Gene Expression Profiling*
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Mitosis / genetics*
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MutS Homolog 2 Protein
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Oligonucleotide Array Sequence Analysis
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Promoter Regions, Genetic / genetics
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Proto-Oncogene Proteins / genetics
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Reverse Transcriptase Polymerase Chain Reaction
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Transcription Factors / metabolism*
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Up-Regulation*
Substances
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Cell Cycle Proteins
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DNA-Binding Proteins
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E2F Transcription Factors
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E2F1 Transcription Factor
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E2F3 Transcription Factor
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E2f1 protein, rat
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Proto-Oncogene Proteins
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RNA, Messenger
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Transcription Factors
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Msh2 protein, rat
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MutS Homolog 2 Protein