Abstract
Transcription of the brain-derived neurotrophic factor (BDNF) gene is regulated in a calcium- and neuron-selective manner; however, the mechanisms that underlie this selectivity are not known. We have characterized a new calcium-response element, CaRE1, that is required for activity-dependent transcription of BDNF exon III and have cloned a transcription factor, CaRF, that activates transcription from BDNF promoter III in a CaRE1-dependent manner. The transcriptional activity of CaRF is regulated in a calcium- and neuron-selective manner, suggesting that CaRF may confer selectivity upon the activity-dependent induction of BDNF exon III expression.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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1-Methyl-3-isobutylxanthine / pharmacology
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Amino Acid Sequence
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Animals
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Brain / cytology
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Brain / metabolism
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Brain-Derived Neurotrophic Factor / genetics
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Brain-Derived Neurotrophic Factor / metabolism*
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Calcium / metabolism
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Cells, Cultured
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Cyclic AMP / analogs & derivatives
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Cyclic AMP / metabolism
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DNA-Binding Proteins
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Exons / genetics
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Gene Expression Regulation / physiology*
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Genes, Reporter
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Humans
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In Situ Hybridization
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Microscopy, Fluorescence
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Molecular Sequence Data
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Neurons / cytology
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Neurons / drug effects
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Neurons / metabolism*
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Nuclear Proteins / metabolism
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Phosphodiesterase Inhibitors / pharmacology
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Rats
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Rats, Long-Evans
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Regulatory Sequences, Nucleic Acid / genetics
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Response Elements / genetics*
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Tissue Distribution
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Transcription Factors / chemistry
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Transcription Factors / metabolism*
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Two-Hybrid System Techniques
Substances
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Brain-Derived Neurotrophic Factor
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CaRF protein, human
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DNA-Binding Proteins
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Nuclear Proteins
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Phosphodiesterase Inhibitors
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Transcription Factors
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Cyclic AMP
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Calcium
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1-Methyl-3-isobutylxanthine
Associated data
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GENBANK/AF454946
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GENBANK/AF454947