Abstract
We show here that seeds from the knockout mutant of the Arabidopsis DAG1 gene encoding a Dof zinc finger transcription factor have an altered response to red and far-red light. Mutant dag1 seeds are induced to germinate by much lower red light fluence rates, and germination reaches more quickly a point where it is independent of phytochrome signaling. Moreover, although microscopic analysis reveals no obvious structural alterations in the seed coat (testa) of dag1 seeds, staining assays with different dyes point to an abnormal fragility of the testa. By extensive in situ mRNA hybridization analysis we show here that the gene, which is not expressed in the embryo, is specifically expressed in the phloem of all organs of the mother plant.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / genetics*
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Arabidopsis / metabolism
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Arabidopsis / radiation effects
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Arabidopsis Proteins*
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Biological Transport / radiation effects
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DNA-Binding Proteins / genetics
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Gene Expression Regulation, Plant / radiation effects
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Germination / genetics
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Germination / radiation effects
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In Situ Hybridization
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Light
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Mutation
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Phytochrome / metabolism
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Plant Proteins / genetics*
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RNA, Plant / genetics
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RNA, Plant / metabolism
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Seeds / genetics*
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Seeds / metabolism
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Seeds / radiation effects
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Signal Transduction
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Transcription Factors / genetics
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Zinc Fingers / genetics*
Substances
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Arabidopsis Proteins
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DAG protein, Antirrhinum majus
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DAG1 protein, Arabidopsis
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DNA-Binding Proteins
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Dof3 protein, Arabidopsis
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Plant Proteins
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RNA, Plant
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Transcription Factors
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Phytochrome